Transient over-expression of E47, E12, FOXO1 or Foxp1A increased RAG expression in MCF-7 cells.

<p>MCF-7 cells were transfected with the empty vector or transcription factor expression vector pIRES2-EGFP-hE47, pIRES2-EGFP-hE12, pcDNA3-GFP-FOXO1;AAA or pCDNAI/NEO-5’HA-Foxp1A. 48 hours later total protein from MCF-7 cells was collected and analyzed by Western blot. GAPDH was shown as a loading control. Experiments were repeated three times with similar results. Similar results were obtained for the A549 cell line (data not shown).