Individual detection of single phosphorylated PDGFRβ and <i>DUSP6</i> molecules in BJhTert cells.

<p>(A) Detection of individual phosphorylated PDGFRβ using <i>in situ</i> PLA in PDGF-BB-stimulated cells. Black circles represent the numbers of RCPs detected in individual cells (in total ∼90–130 cells per condition), the black bar represents the median of the population and grey boxes the 25% and 75% quartiles. The experiment is a representative example of three replicate experiments. (B) Detection of individual <i>DUSP6</i> mRNA molecules using padlock probes in BJhTert cells stimulated with PDGF-BB for different length of time. Black circles represent the numbers of RCPs detected in individual cells (in total ∼70–180 cells per condition), the black bar represents the median of the population and grey boxes the 25% and 75% quartiles. The experiment is a representative example of three replicate experiments.