Whole-cell patch clamp of cAMP-evoked Cl⁻ current in human peripheral macrophages.

<p>(A) representative currents in basal condition (basal) or in response to the administration the cAMP-containing cocktail (cAMP), recorded on voltage-clamped macrophages. cAMP-evoked currents were blocked in the presence of the specific inhibitor CFTR_inh-172 (10 µM), added to the bath solution (cAMP/CFTR_inh-172). Currents were recorded in 200 ms voltage steps from −110 to +110 mV with 10 mV increments from a holding potential of −40 mV. (B) Averaged current/voltage relationship in basal conditions (basal, n = 9), in the presence of a cAMP-containing cocktail (cAMP, n = 16) or cAMP-containing cocktail plus 10 µM CFTR_inh-172 (cAMP/CFTR_inh-172, n = 7). (C) Current densities obtained at +110 mV in the three described experimental conditions, in parentheses the number of recorded cells are shown. Data reported in panel (B) and (C) are means±S.E.