Effect of 8-pCPT-cAMP on bTREK-1 currents in perforated patch recordings

(A and B) Perforated patch recordings: whole cell K currents were recorded in the nystatin perforated patch configuration in response to voltage steps applied at 30-s intervals from a holding potential of −80 to +20 mV with (right traces) or without (left traces) depolarizing prepulses. After bTREK-1 reached a stable amplitude, cells were superfused with 8-pCPT-2′-O-Me-cAMP (designated EA for Epac activator) (30 μM), ACTH (200 pM), or 8-pCPT-cAMP (30 or 300 μM), as indicated. Numbers on current traces correspond to those on plot at right. (C) Summary of experiments as in A and B. Bars indicate mean ± SEM of indicated number of determinations.<p><b>Copyright information:</b></p><p>Taken from "ACTH Inhibits bTREK-1 K Channels through Multiple cAMP-dependent Signaling Pathways"</p><p></p><p>The Journal of General Physiology 2008;132(2):279-294.</p><p>Published online Jan 2008</p><p>PMCID:PMC2483331.</p><p>