NaHS inhibited TNF-α-induced p38 phosphorylation.

<p>(A) HUVEC were stimulated with TNF-α (10 ng/ml) for indicated periods. The total and phosphorylation levels of MAPK were measured by Western blot. The experiment was repeated 3 times with equal results. Cells were incubated with indicated concentrations of NaHS for 30 min, then treated with TNF-α (10 ng/ml) for another 15 min. Phosphorylation levels of p38 (B), JNK1/2(<b>C</b>) and ERK1/2 (D) were analyzed by Western blot. ^#<i>P</i><0.05 compared with unstimulated cells, *<i>P</i><0.05 compared with TNF-α-stimulated cells. Data are the mean ± S.E.M of results from at least three independent experiments, each performed in duplicate.