Biotinylated K_V10.1-AP is detected in the inner nuclear envelope after labeling of the plasma membrane channel.

<p>After enzymatic biotinylation, cytoplasmic and nuclear extracts were pulled down (A) with streptavidin-magnetic beads. K_V10.1-AP was detected in the nuclear fraction and resistant to detergent extraction. As a control, (B) shows the total K_V10.1-AP and LUMA signals from these extracts before pull-down of biotinylated K_v10.1-AP. Densitometry for each lane is represented below the corresponding blot (mean±s.e.m, n = 3).