KPNA mediates the nuclear translocation of PHB2.

<p>(A) Immunoblotting analysis was performed to detect the subcellular localization of KPNA, ERα and PHB2. COS-7 cells co-transfected with HA-PHB2, each FLAG-KPNA and FLAG-ERα were treated with 10 nM E2 for 24 h and separated into cytoplasmic and nuclear fractions. Each KPNAs and ERα were detected by endogenous antibody. α/β-Tubulin (tubulin) and lamin B1 (lamin) were used as loading controls for the cytoplasmic (Cyto) and nuclear (N) fractions, respectively. (B) Representative immunofluorescence images of the subcellular localization of HA-PHB2 in COS-7 cells are shown; HA-PHB2 (red), DAPI (blue). (C) Statistical analyses of the nuclear intensity of translocated PHB2. The data represent the mean ± SD of four different points (***<i>P</i><0.001 in a two-sided Student’s <i>t</i>-test).