Expression of 2A^pro provokes nuclear-cytoplasmic redistribution of splicing factors.

<p>(A) Huh7-T7 cells were transfected with pTM1-2A, pTM1-2AM, PV-Rep or empty plasmid. Subcellular distributions of the indicated proteins were analyzed by immunoblot using the respective antibodies. <i>T</i> refers to total, <i>N</i> refers to nuclear, and <i>C</i> refers to cytoplasmic fractions. GAPDH and Ref1 were used as controls for cytoplasmic and nuclear location respectively. (B) The densitometry of the bands was used to calculate the cytoplasm/nucleus ratio for each protein shown. Data are mean ± s.d. (n = 3; *<i>P</i><0.05; **<i>P</i><0.01 by Student’s <i>t</i>-test). (C-E) Immunofluorescence staining of TIA1, TIAR and HuR proteins in Huh-7-T7 cells. Cells transfected as in (A) were immunolabelled with the indicated antibodies. Merge/Topro-3 refers to simultaneous visualization of images. Scale bars: 10 µm.