Gli2 activates the Ascl1 promoter.

<p>HEK-293 cells were transiently cotransfected with or without Gli2 and a construct containing the Ascl1 8 kb promoter driving the luciferase gene (Ascl1-luc) in ratios 2∶1, 4∶1 and 6∶1 relative to Ascl1-luc. Equal parts of Gli/EnR were transfected together with Gli2 at a ratio of 4∶1 relative to Ascl1-luc. Fold changes are relative to Ascl1-luc activity with the Ascl1-luc plasmid alone. Error bars represent +/− SEM from three biological replicas (*p<0.05, **p<0.01). No significant (n.s.) increase was observed in the presence of Gli/EnR.