<i>DUT1</i> expression levels and dUTPase activities in RNAi/<i>DUT1</i> lines compared to control plants.

<p>A - <i>DUT1</i> expression level was analyzed by quantitative real time RT-PCR in young leaves of RNAi/<i>DUT1</i>-4 and -5 compared with WT plants (Ws ecotype). The relative mRNA normalized to <i>ACTIN</i> is reported. Error bars correspond to standard deviations. B - Level of dUTPase activity in RNAi/<i>DUT1-</i>4 and -5 plant lines compared to control plant (empty vector, RNAi/0). Activity was determined by measuring the dUMP produced by UPLC, after supplementation of plant extract with dUTP at 30°C for increasing lengths of time (three points in two independent experiments). Error bars correspond to standard deviations.