Transcript levels of target genes in transgenic T₁ wheat RNAi plants and the non-transgenic wheat control.

<p>(A) Comparison between the average of five transgenic plants (RNAi) and the wild type control using contrasts. (B–G) Comparison between individual transgenic lines and wild type using Dunnett’s test. (B) <i>FT1</i>, (C) <i>FT2</i>, (D) <i>FT3</i>, (E) <i>FT4</i>, (F) <i>FT5</i>, (G) <i>VRN1</i> (gene regulated by <i>FT1</i>). <i>ACTIN</i> was used as the internal control. Samples were harvested at 4∶00 p.m., when the wild type controls began to flower. Asterisks indicate <i>P</i> values: * = <i>P</i><0.05, ** = <i>P</i><0.01, *** = <i>P</i><0.001.