BSO treatment inhibits adipogenesis.

<p>A and B: Confluent 3T3-L1 cells were treated with different doses of BSO for 2 days and induced to differentiate. BSO treatment was continued until day 7 of differentiation. Differentiated cells were stained with Oil-red-O and absorbance was measured at 510 nm using a spectrophotometer. C: Confluent 3T3-L1 cells were treated with 100 µM BSO for 2 days and induced to differentiate. BSO treatment was continued until day 7 of differentiation. On day 7 mRNA was harvested and aP-2 expression was analyzed by real-time RT-PCR. D: Confluent subcutaneous stromal vascular cells were treated with 10 µM BSO. After 2 days cells were induced to differentiate and treated with BSO until day 7 of differentiation. On day 7 mRNA was harvested and aP-2 expression was analyzed. E: 3T3-L1 cells were treated with 100 µM BSO for 2 days and induced to differentiate. BSO treatment was continued until day 2 or day 7 of differentiation as indicated. On day 7 cells were stained with Oil-red-O and absorbance was measured. All results are presented as mean ± SEM (* p<0.05 vs. control).