Resveratrol modulates adipogenic differentiation of hMSCs in a dosage dependent manner.

<p><b>A</b>). Oil droplets were stained by Oil-Red-O solution and subsequently quantified under three different treatment schemes: concurrent treatment, pretreatment and equal density. <b>B</b>). Images of Oil-Red-O stained cells under different treatment conditions. Concurrent treatment: cells were exposed to both resveratrol and AIM throughout the differentiation duration; Pretreatment: cells were cultured in BM/resveratrol conditioned media continuously for certain days prior to AIM induction; Equal density: Cells were cultured in BM/resveratrol conditioned media continuously for certain days and re-plated at equal density before AIM induction. Except for the pretreatment groups, all experiments were repeated 3 times independently, with triplicates in each experimental set. A representative data set is presented for each group and data shown are the relative mean values of triplicates normalized to the mean value of the BM control cells in each group. Error bars represent standard deviation. *: p<0.05 vs. BM. Images were taken at 8× except for bottom row in B) (6.3×).