Effects of podocalyxin silence on cell morphology and podocalyxin production.

<p><b>A</b>): Podocalyxin shRNA transduced cells (PODXL-KD) (lane 2,4,6,8) and control A549 cells (transduced with the same vector without shRNA insert) (lane 1,3,5,7) were cultured in the presence or absence of TGF-β for 1 to 3 days. Podocalyxin levels were examined by western blot. GAPDH were also determined as loading controls. <b>B</b>): Cell morphology under phase contrast microscope before and after silencing podocalyxin. Scale bar = 50 µm.