Podocalyxin is required for TGF-β induced EMT marker expressions.

<p>A549 and PODXL-KD cells were treated with TGF-β for 1–3 days, and monitored by western blot for their expressions of podocalyxin, E-cadherin and vimentin. TGF-β up-regulated the production of podocalyxin and vimentin in A549 cells, and down-regulated E-cadherin level to zero in a time dependent manner. Silencing of podocalyxin interfered with TGF-β mediated down-regulation of E-cadherin and up-regulation of vimentin.