The ability of KLK6 to decrease death of murine splenocytes relates to its ability to delay the apoptotic cascade.

<p>(A) Histogram and corresponding dot plots (B) demonstrate that KLK6 significantly reduced the percentage of dead cells (Annexin V-FITC⁺ and PI⁺) observed in cultures of splenocytes under resting conditions and after exposure to dexamethasone (0.1 µM), or staurosporine (1 µM). Reduced cell death was accompanied by a significant increase in the live population (unlabeled cells) under resting conditions and with exposure to staurosporine. KLK6 exposure also resulted in a corresponding increase in the percentage of early apoptotic cells (Annexin V-FITC⁺, PI^-) in the presence of apoptosis inducing agents. Data shown are the mean ± SEM of triplicate cultures examined in parallel; One Way ANOVA with SNK post hoc test for multiple comparisons, P<0.001***, P<0.003**, P<0.007*. All results shown are representative of that seen in at least 3 independent experiments.