Six-color FACS analysis of lymphocytes derived from CSF and peripheral blood.
- Benedikt Fritzsching (354598)
- Jürgen Haas (29229)
- Fatima König (354599)
- Pierre Kunz (354600)
- Eva Fritzsching (354601)
- Johannes Pöschl (354602)
- Peter H. Krammer (33078)
- Wolfgang Brück (251206)
- Elisabeth Suri-Payer (354603)
- Brigitte Wildemann (316416)
Publication date
February 2013
DOIAbstract
<p>Analysis of CSF and PBMC from 17 treatment-naïve MS patients. Isolated lymphocytes were stained with monoclonal antibodies against CD4, CD25, CD127, FOXP3, CD45RO and CD95. <b>(A)</b> Lymphocytes were gated for Treg and the percentage of CD95<sup>hi</sup>CD45RO<sup>hi</sup> was determined. Treg were increased in CSF when compared to peripheral blood, * indicates p<0.05. <b>(B)</b> Expression of CD95 on Treg from CSF and PBMC derived from the same MS patient on the same day, representative figure.</p
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