<p>Chemotactic indices (CI) of Treg from a healthy donor towards CSF supernatants of 11 MS patients as determined by transwell migration assays. Original input PBMC and migrated cells were stained for CD4, CD25, FOXP3, CD95, CD45RO, and CD45RA, and CIs were calculated by dividing percentages of Treg subsets in CD4<sup>+</sup> T-cells of migrated cells by percentages of Treg in CD4<sup>+</sup> T-cells in original input PBMCs. Symbols indicate CIs of individual patients. Means are indicated, ** indicates p<0.001.</p
<p>(A) Quantification of the TSDR demethylation levels of the <i>foxp3</i> promoter in freshly FACS ...
<p>Scatter plots from three representative subjects, one healthy donor (HC; <b>A</b>), a stable MS p...
Regulatory T cell (Treg) therapy is a promising strategy to induce transplant tolerance and may perm...
<p>Analysis of CSF and PBMC from 17 treatment-naïve MS patients. Isolated lymphocytes were stained w...
<p>(A) A representative flow cytometry analysis of Treg subpopulations gated by CD4<sup>+</sup> T ce...
<p>(A) We defined Teff as Treg-depleted PBMC stimulated with anti-CD3 mAb. Teff from HC were cocultu...
<p>Representative flow cytometry analysis of CD25<sup>+</sup>CD127<sup>low</sup> subpopulations amon...
<p>(<b>A</b>) Transmigration Assay of CD4<sup>+</sup> T cells from healthy donors (HDs; <i>n</i> = 5...
<p>PBMC from JDM patients (JDM) with active (active) or remitting (remission) disease, and controls ...
<p>The percentage of FoxP3<sup>+</sup> cells in the CD4<sup>+</sup>CD25<sup>high</sup> gate is shown...
<p>Representative contour plots of Tregs (A) (CD25<sup>+</sup>Foxp3<sup>+</sup> of CD4<sup>+</sup> c...
<p>Based on the expression of CD45RA and FOXP3, five subsets of CD4+T cells are defined: FOXP3<sup>l...
<p>PBMCs were isolated from individual subjects and stained with different fluorescent antibodies. T...
<p>(A) Flow cytometry analysis of purified Treg stained with anti- CD4, CD25, FOXP3 and CD45RA antib...
<p>(A) PBMCs were stained with Abs against CD4, CD45RA, and FOXP3, and then analyzed by flow cytomet...
<p>(A) Quantification of the TSDR demethylation levels of the <i>foxp3</i> promoter in freshly FACS ...
<p>Scatter plots from three representative subjects, one healthy donor (HC; <b>A</b>), a stable MS p...
Regulatory T cell (Treg) therapy is a promising strategy to induce transplant tolerance and may perm...
<p>Analysis of CSF and PBMC from 17 treatment-naïve MS patients. Isolated lymphocytes were stained w...
<p>(A) A representative flow cytometry analysis of Treg subpopulations gated by CD4<sup>+</sup> T ce...
<p>(A) We defined Teff as Treg-depleted PBMC stimulated with anti-CD3 mAb. Teff from HC were cocultu...
<p>Representative flow cytometry analysis of CD25<sup>+</sup>CD127<sup>low</sup> subpopulations amon...
<p>(<b>A</b>) Transmigration Assay of CD4<sup>+</sup> T cells from healthy donors (HDs; <i>n</i> = 5...
<p>PBMC from JDM patients (JDM) with active (active) or remitting (remission) disease, and controls ...
<p>The percentage of FoxP3<sup>+</sup> cells in the CD4<sup>+</sup>CD25<sup>high</sup> gate is shown...
<p>Representative contour plots of Tregs (A) (CD25<sup>+</sup>Foxp3<sup>+</sup> of CD4<sup>+</sup> c...
<p>Based on the expression of CD45RA and FOXP3, five subsets of CD4+T cells are defined: FOXP3<sup>l...
<p>PBMCs were isolated from individual subjects and stained with different fluorescent antibodies. T...
<p>(A) Flow cytometry analysis of purified Treg stained with anti- CD4, CD25, FOXP3 and CD45RA antib...
<p>(A) PBMCs were stained with Abs against CD4, CD45RA, and FOXP3, and then analyzed by flow cytomet...
<p>(A) Quantification of the TSDR demethylation levels of the <i>foxp3</i> promoter in freshly FACS ...
<p>Scatter plots from three representative subjects, one healthy donor (HC; <b>A</b>), a stable MS p...
Regulatory T cell (Treg) therapy is a promising strategy to induce transplant tolerance and may perm...