Simvastatin decreases eNOS expression.

<p><b>A.</b> Representative images of LacZ staining of the middle cerebral artery (MCA) from naïve, vehicle, pre- and post-SAH simvastatin-treated animals. A thick densely stained LacZ positivity appears in the endothelial layer of the MCA from vehicle animals (arrow heads). This is significantly reduced in both pre- or post-SAH treatment groups, to a similar level in naïve group. Scale bar is 50 µm. <b>B.</b> Quantification of phosphorylated eNOS from naïve, vehicle or simvastatin pre-SAH treated groups. Simvastatin treatment significantly reduced phosphorylated eNOS almost to the same level in the naïve animals. A representative blot is shown for each group. <b>C</b> and <b>D</b>. Quantification of phosphorylated eNOS from pre-SAH or post-SAH treated groups, respectively. Both pre- and post-SAH treatment significantly reduced phosphorylated eNOS expression (<i>P</i><0.01 for pre-treatment, <i>P</i><0.05 for post-treatment). A representative blot is shown for each treatment group under corresponding bar graphs. All data are mean ± SD.