Intracellular growth of <i>Salmonella Typhimurium</i> in IPEC-J2 cells after post-incubation with <i>E. coli</i> Nissle 1917.

<p>Confluent monolayers of IPEC-J2 cells were infected with <i>Salmonella Typhimurium</i> for one hour using an MOI of 1∶1 <i>Salmonella</i> to host cells. After one additional hour of incubation in media containing gentamicin, IPEC-J2 cells were incubated with <i>E. coli</i> Nissle 1917 (EcN), <i>E. coli</i> 140815 or <i>E. coli</i> MG1655 for two hours using an MOI of 100∶1 <i>E. coli</i> to host cells, followed by incubation in media with gentamicin. Intracellular <i>Salmonella</i> numbers are presented per well of a 24-well plate. The data are the mean ± S.E.M. of at least three separate experiments in duplicate wells.