Intracellular growth of GlmS⁻ mutant <i>S. typhimurium</i>.

<p>(A) 5×10⁵ CFU of GlmS⁻ mutant (SKS1001) and wild-type (SCH2005) <i>Salmonellae</i> grown in LB supplemented with 0.2% GlcNAc was mixed with HeLa cells. Gentamycin-resistant intracellular <i>Salmonellae</i> were enumerated by determining the number of CFU at the indicated times. (B) The intracellular GlmS⁻ mutant bacteria, GlmS⁻ mutant bacteria carrying <i>^S.tGlmS⁺p</i>, and wild-type bacteria were enumerated at 0 and 24 hrs in HeLa cells (B) and peritoneal macrophages (C).