Hyperinsulinemic-euglycemic clamps.

<p>Third intracerebroventricular leptin treatment (black bars) significantly improved hepatic insulin sensitivity and glucose disposal during hyperinsulinemic-euglycemic clamps in male MKR mice relative to artificial cerebrospinal fluid (ACSF) vehicle treated controls (open bars) and pair fed, ACSF treated male MKR mice (hatched bars). A. Glucose infusion rate (GIR) during the clamp, B. peripheral glucose disappearance rate (Rd), hepatic glucose production (GP) under basal conditions (C) or hyperinsulinemia (D). All data presented as means ± SEM. N = 5–6/group. Different superscript letters indicate significant differences at p<0.05.