Effects of GSK101 on whole-cell membrane current (patch clamp) in HeLa-TRPV4 cells.

<p>A–C: HeLa-TRPV4 cells in Ca²⁺ containing media. A. A representative trace of GSK101-induced whole cell current showing a rapid increase at 1 min stimulation followed by channel desensitization (holding potential at −90 mV). B<b>.</b> Summary data showing the time course of GSK101-induced membrane current (Mean ± SE, n = 4) displaying a peak activation at 1 min followed by a partial desensitization of the channels. The time course for desensitization was fit to a single exponential equation and yielded a time constant (τ) for decay of 0.8±0.1 minutes.C<b>.</b> Current-voltage plot of a HeLa-TRPV4 cell under GSK101 stimulation, using a voltage ramp protocol from −100 to 100 mV over 200 msec. <b><i>D–E</i></b>: HeLa-TRPV4 cells in Ca²⁺ free media (Na⁺ containing media). D. A representative trace of GSK101-induced whole cell current showing a similar pattern of channel activation as shown in A, but at a larger magnitude in Ca²⁺ free media. E. Summary data showing the time course of GSK101-induced membrane current (Mean ± SE, n = 4) in Ca²⁺ free media. The time course for desensitization was fit to a single exponential equation witha τ for decay of 3.8±1.8 minutes (NS from Ca²⁺-containing media in B). F. Current-voltage plot of a HeLa-TRPV4 cell under GSK101 stimulation in Ca²⁺ free media, using a voltage ramp protocol from −100 to 100 mV over 200 msec.