Nestin-GFP+ cells attached to myofiber (satellite cells) do not produce Tuj1+ cells with a neural phenotype.

<p><b>A.</b> A representative EDL muscle cross-section from a nestin-GFP transgenic mouse showing GFP expression and Pax7+ immunoreaction (n = 3 mice, 6 EDL muscles). Myofibers were counterstained with Hoechst. The merge image shows examples of GFP+/Pax- cells (arrows). Scale bar = 50 µm. <b>B.</b> Enzymatically dissociated single FDB muscle fiber showing 2 nestin-GFP+ satellite cells that immunoreact to Pax7 and overlap with Hoechst nuclear staining. Scale bar = 100 µm. <b>C.</b> FDB satellite cell time-lapse analysis. A nestin-GFP+ satellite cell attached to an FDB fiber (arrow) analyzed for more than 6 days. Snapshots of the complete record at 4, 50, 80, 154, and 162 h. Brightfield (a, c, e, g, h) and fluorescence (b, d, f). Fluorescence completely disappeared at 162 h in culture. Scale bar = 50 µm. <b>Da.</b> Four-month satellite cell culture from isolated EDL fibers. Myoblasts and myotubes are stained for desmin and Hoechst, which overlap with the brightfield image. <b>b.</b> The culture shown in <i>Da</i> is MyoD+ but Tuj1-. Scale bar for all pictures in D = 100 µm.