Co-expression of Zfp568 with Nestin but not Tuj1 or MAP2 during neural differentiation of the mouse ES cells.

<p>Mouse ES cells were plated at low density in the ES cell medium containing serum and LIF, and then transferred to N2B27 without LIF after overnight incubation (Day 0). (<b>A</b>) Co-staining patterns of Zfp568 with the ES cell markers Oct4 and Sox2, respectively, of ES cells in the ES medium. (<b>B</b>) Co-staining patterns of Zfp568 with the neural stem cell marker Nestin and neuronal markers, Tuj1 and MAP2, on different days in the N2B27 medium. The percentages of single- and double- stainings, as listed under each panel, were each calculated by scoring the cells in at least three random chosen fields from two independent sets of experiments. (n = 10 for Day3, n = 7 for Day 7, n = 7 for Day 9). Bar, 50 µm.