Neuromuscular junctions of <i>Cra1</i>/+ mice are abnormal at symptomatic ages.

<p>A) α-bungarotoxin labeled NMJs (Scale bar = 20 µm) B) Quantification of α-bungarotoxin labeling demonstrates no differences in the number of NMJs per section (N = 3 animals per genotype). C) Quantification of α-bungarotoxin labeled NMJs demonstrates no difference in total area (as measured by delineating outer edge and measuring area within that boundary, area analyzed is within white trace of 4Ai) of NMJs in <i>Cra1</i>/+ mice (N = 3 animals per genotype). D) NMJ complexity, as measured by the synaptic area of α-bungarotoxin label (4Aii), is significantly decreased in 2 month and 6 month old <i>Cra1</i>/+ mice (*P<0.05; N = 3 mice per genotype).