Quantification of neuromuscular junction (NMJ) innervation at 60days in SOD1^G93A transgenic mice.

<p>(A, B) Immunofluorescence staining of gastrocnemius muscle with α-Bungarotoxin (red) and anti-neurofilament antibodies (green). NMJ were counted as innervated when end-plates showed overlap of neurofilament and AChR staining (A, image from a non-transgenic mouse) and denervated when endplates showed absence of neurofilament labeling (B image from a SOD1G93A transgenic mouse). Scale bar 50 µM. The percentage of α-Bungarotoxin stained end-plates showing complete and partial co-localisation of neurofilament staining versus absence of colocalisation (C) is shown for 5 non-transgenic mice (NTG) and 5 SOD1G93A transgenic mice (G93A) at 60 days of age. Average +/−standard deviation is shown (NTG 96.4+/−2.5%; G93A 37.4+/−8.5%, p<0.0001, Student's T test). Graph displays result for individual animals. Median horizontal line indicates the mean value. Upper and lower horizontal lines linked by a vertical line show SD.