Ag-specific IgG isotype responses in mice vaccinated with different vaccine regimens in short-term protected group (ten days post boost) before and 3 months after <i>L. donovani</i> challenge infection.

<p>Ten days after final immunization and 3 months after challenge infection, blood serum samples were collected and assayed for IgG2a, IgG1, and IgG2a:IgG1 by ELISA. The results are shown as the mean absorbance values ± S.E. of five individual mice per group, representative of two independent experiments with similar results. <i>p</i> values were calculated using one-way ANOVA and Tukey's multiple comparison test.</p>a<p>Significantly higher than gp63 DNA either free or in presence of CpG (<i>p</i><0.001).</p>b<p>Significantly higher than DNA-prime/Protein-boost (<i>p</i><0.001).</p>c<p>Significantly higher than gp63 DNA (<i>p</i><0.05).