DALIS are detectable by immune fluorescence and a filter trap assay.

<p>(A) Following addition of LPS to RAW309 macrophages and mouse BMDCs ubiquitin conjugates were detected by immune fluorescence using the FK2 antibody. Transient DALIS formation peaks around 12 h after LPS addition. Scale bars: 10 µm. (B) Immune cells were lysed at the indicated time points during LPS-induced maturation in detergent containing buffer and passed through a nitrocellulose filter. Detergent insoluble ubiquitin conjugates were detected on the filter with the FK2 antibody. 50 µg of cell lysate were passed through each dot.