Binding of EV71 VLPs to immature human monocyte-derived DCs.

<p>For the analysis of concentration-dependent effects, human monocyte-derived DCs were incubated with 1 or 10<i> </i>µg/mL EV71 VLPs for 30<i> </i>min on ice to allow binding to the cell surface. For the analysis of time-dependent effects, DCs were incubated with 5<i> </i>µg/mL of EV71 VLPs for 30 or 120<i> </i>min on ice. Detection was facilitated using an anti-EV71 Ab and anti-mouse-IgG Ab (labeled with Alexa Fluor 488). Levels of fluorescence were determined using immunofluorescence confocal microscopy (A) and FACS analysis (B, C). (B) and (C) indicate the respective concentration-dependent and time-dependent effects of the binding of EV71 VLPs to immature human monocyte-derived DCs. The control cells (no VLPs; filled histogram) were stained with the same primary antibody (anti-EV71 Ab) followed by a secondary antibody. (D) and (E) indicate the bar diagram of mean fluorescence intensity (MFI) with statistics for concentration- and time-dependent binding of EV71 VLPs to DCs. Representative data from three different donors with similar results are shown. Values represent the means ± SEs of triplicate samples, and asterisks indicate statistically significant differences (*, <i>P</i><0.05).