Summary and location of mutation sites.

<p><b>A:</b> Summary of mutation sites in the amino acid sequence of all the mutants. The full sequence is divided into three parts by two dashed lines. Each part represents a separate domain of irrE (from left to right: N-terminal domain, HTH domain, C-terminal domain). <b>B:</b> Location of mutation sites of all the mutants in a modeled structure of irrE from <i>D. radiodurans</i>. Domains of irrE: N-terminal domain, cyan; HTH domain, green; C-terminal domain, pink. Mutation sites of: ethanol-tolerant mutants, yellow; butanol-tolerant mutant, blue; acetate-tolerant mutant, red.