A Antigen ELISA using anti-MUC1 scFv-Fc fusion proteins and anti-MUC1 IgGs.

<p>50 µg MUC1 peptide antigen (32mer cys) were immobilised in each well. Antibodies were incubated in different concentrations. A goat-anti-human-IgG (Fc spec.) HRP conjugate (1∶10,000) was used for detection of bound anti-MUC1 antibodies. B Flow cytometry analysis of anti-MUC1 IgG and scFv-Fc antibodies titrated on T47D. Antibodies were detected with goat anti-human IgG Alexa488 conjungate (1∶200) (Invitrogen, Darmstadt, Germany). 10000 cells were analysed per run. MFI was plotted against the antibody concentration.