T-DNA insertion mutant morphologies of V592 and insertion identification.

<p>(A) Colony morphologies of wild type V592 (a) and T-DNA insertion mutants (b to f), classified as intermediate type (b), hyphal type (c) and velum type (d), as well as special types such as red pigment (e) and slow-growing rate (f) mutants. (B) Identification of T-DNA insertional copy numbers. Genomic DNA isolated from one hundred randomly selected transformants was digested with <i>Xba</i>I for DNA gel blot analysis. Representative result with twenty mutants is shown. Hybridization was performed with ³²P-labeled EGFP-specific DNA probe as shown at right.