Verification of clonal purity of five <i>E</i>. <i>chaffeensis</i> mutants by Southern blot analysis.

<p>A) Predicted Bgl II digested genomic DNA fragments calculated based on the insertion locations (orientation of the insertions is represented by the arrowheads). B) Southern blot data identifying the expected DNA fragments; lanes Wt and 1–5 represent genomic DNAs derived from <i>E</i>. <i>chaffeensis</i> wild-type and mutants Ech_0202, Ech_0284, Ech_0379, Ech_0480 and Ech_0660, respectively. Molecular weights of the DNA markers migrated at specific locations were presented at the left end of panel B. Expected size genomic DNA fragments for each mutant were identified on the right end of panel B.