Effects of different preservation media on spindle configuration in porcine oocytes after IVM.

<p>A. a, represents a normal spindle in porcine oocyte. b and c represent abnormal spindle. In b1, c1 arrows indicate abnormal spindle organization distribution. In b2, arrows indicate fragmented and displaced chromosomes. Green, tubulin; Red, chromatin. Scale bar = 10 µm. B. Normal ratios of spindle configuration in porcine oocytes after preservation in different media following IVM. Porcine oocytes were preserved in TCM-199, pFF and FCS at 27.5°C for 3 d, then spindle was stained with FITC-α-tubulin after culture for 44 h <i>in vitro</i>. Normal and abnormal spindle configuration were detected. The graph shows the mean ± SEM of three independent experiments. The number “n” over the bars means the total treated oocyte in every group. The superscripts ^{a, b} over the bars represent values of normal tubulin configuration that differ significantly between groups (P<0.05).