Treatment with NAM and Sirtinol induces meiotic defects in porcine oocytes.

<p>(A) Meiotic spindle morphology in second meiosis (MII) oocytes. Control oocytes had barrel-shaped spindles and well aligned chromosomes, whereas spindle defects were frequently observed in NAM and Sirtinol-treated oocytes. Moreover, chromosomes displayed misalignment in treatment oocytes. Green, Tubulin; blue, chromatin. Bar = 20μm (B) Quantification of spindle defects and chromosome misalignment in control and inhibitor-treated oocytes. The percentage of abnormal spindle morphology was significant higher in the NAM- and Sirtinol-treated oocytes than in the control oocytes. *, significant, <i>p</i> < 0.05.