GW4064 treatment inhibited ET-1-induced phosphorylation of MLC in activated HSCs.

<p>Activated HSCs were treated with DMSO or 1 µmol/L GW4064 for 18 hours. ET-1 (10 nM) was then added to the medium, and the cells were harvested 10 min later. Phosphorylation of MLC was detected by Western using appropriate phospho-specific antibody. An immunoblot of total MLC present in the cell extracts is shown as a loading control (lower panel). A representative immunoblot from three independent experiments is presented.