Expression of Myc mutants in Rat1a cells.

<p>(<b>a</b>) Diagram of the Myc protein. The approximately 150 residue TRD is shaded, with an expanded MBII domain and relevant amino acids substitutions depicted below the diagram. The basic-helix-loop-helix dimerization domain at the extreme C-terminus of the protein is indicated by the checkered box. (<b>b</b>) Each of the indicated mutations, along with wtMyc was expressed in Rat1a fibroblasts following lentiviral-mediated transduction. An empty lentiviral vector infection served as a negative control. Pooled, blasticidin-resistant colonies were subjected to western analysis for either Myc or β-tubulin, which served as a loading control. The monoclonal antibody used to detect human Myc proteins had some cross reactivity with endogenous rat Myc.