Photographs of goat oocytes after different treatments to induce ooplasmic protrusions.

<p>A and A' are the same oocyte observed under phase contrast and fluorescence microscope, respectively, after Hoechst staining. B to K are confocal micrographs, of which, B to E and H to K are merged images with DNA colored blue, α-tubulin green and actin red while F and G with DNA colored red while the activated MAD2 green. A and B show oocytes with protrusions while C shows an oocyte without protrusion after demecolcine treatment. D and E are oocytes with protrusions after treatment with MG132 and caffeine, respectively. F and G are oocytes MAD2-positive and -negative after demecolcine and MG132 treatment, respectively. H and I show D+U- and M+U-treated oocytes with spindles disassembled and intact, respectively. J and K are OA.5h oocytes without protrusion and OA4h oocytes with protrusion, respectively, both showing actin enrichment. Arrow: intact or disintegrated spindles; *: Pb1. The scale bar is 30-µm in A–E and H–K but 70-µm in F and G.