TUNEL staining showing that CQ treatment leads to less apoptotic neurons in the gerbil hippocampal CA1 region.

<p>(<b>A–C</b>) The TUNEL-positive cells in the hippocampal CA1 region were examined at 3 d after ischemia. Compared with sham controls (A), more TUNEL-positive apoptotic neurons were observed in vehicle-treated ischemic gerbils (B), while the number of TUNEL-positive cells was markedly reduced in ischemic gerbils treated with CQ (C), compared with vehicle-treated gerbils. Scale bar = 25 µm. (<b>D</b>) TUNEL-positive neurons in the CA1 region were counted under a light microscope. Statistically significant reduction in the number of TUNEL-positive apoptotic neurons in the CA1 region were found in CQ-treated gerbils, compared with vehicle-treated controls. ** <i>p</i><0.01 (n = 6).