Increased gastric Cyclin D1 expression, and neoplasia, in <i>Kcne2</i>^−/− mice.

<p>A. Western blots of lysates from 3 stomachs per genotype from <i>Kcne2</i>^−/− and <i>Kcne2</i>^+/+ mice, using anti-Cyclin D1 (upper) and anti-GAPDH (lower) antibodies. B. Cyclin D1 immunoreactivity at the base of <i>Kcne2</i>^+/+ oxyntic glands (<i>left</i>) versus more widespread, and nuclear, staining in the mucosa of <i>Kcne2</i>^−/− mice (right). Scale bars: 70 µm. C. Higher magnifications of Cyclin D1 immunohistochemistry panels in B. <i>Left</i>, <i>Kcne2</i>^+/+; <i>right</i>, <i>Kcne2</i>^−/−. Scale bars: 40 µm. D. Serosal (<i>left</i>) and mucosal (<i>right</i>) views of 12-month-old <i>Kcne2</i>^+/+ (upper) and <i>Kcne2</i>^−/− (lower) mouse stomachs. <i>Arrows</i>: <i>left panel</i>, cystic changes in the gastric serosa; <i>right panel</i>, pyloric polypoid adenomas extending into the duodenum. E. <i>Left</i>, H&E-stained section showing a thrombus composed of fibrin and glandular epithelium in a thin-walled vessel within the submucosa of a 12-month-old <i>Kcne2</i>^−/− mouse (scale bar, 300 µm). <i>Right</i>, expanded view of thrombus from boxed region in left panel (scale bar, 30 µm). F. CD34 staining of endothelial cells in the thin-walled vessel shown in panel E (brown staining, red arrows). Gray arrow, glandular epithelium. Scale bar, 50 µm. G. An H&E-stained section from the thin-walled vessel in panel F, illustrating the vessel wall (red arrow), and invading glandular epithelium (gray arrow) with region of fibrin adhesion (black arrow). Scale bar, 50 µm. H. Internal control for CD34 staining, showing a blood vessel from the same <i>Kcne2</i>^−/− mouse stomach as in panels E–G, but without glandular epithelial invasion, stained with anti-CD34 antibody (brown staining, red arrow). Scale bar, 50 µm.