Characterization of alpha and beta cell fractions.

<p>A: Representative image of the beta cell fraction stained for insulin (red) and glucagon (green). B: Representative image of the alpha cell fraction stained likewise. C: Insulin secretion from sorted beta cells. Data are displayed as mean + SEM (islets from separate donors, n = 6 for all glucose concentrations and 5 for KCl). Statistical significance was assessed by one-tailed paired Student's T test comparing stimulated to basal secretion each time.