Primers.

<p>Sites for restriction enzymes BamHI (GGATCC), XhoI (CTCGAG), are shown underlined. Stop codons are shown in bold, in the 3′ to 5′ (TTA) orientation. The third column indicates which peptide fragments each primer was used to construct (See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0011047#pone-0011047-g001" target="_blank">Figure 1</a>). Primers used only for sequencing are indicated by the abbreviation “seq.”