Primers.

<p>Sites for restriction enzymes BamHI (GGATCC), XhoI (CTCGAG), XbaI (TCTAGA) and EcoRV (GATATC) are shown underlined. Stop codons are shown in bold, either in the 5′ to 3′ (TAA) or 3′ to 5′ (TTA) orientation. The third column indicates which peptide fragments each primer was used to construct, or where the primer was used to clone the heavy and light chain variable regions of F1-40. Primers used only for sequencing are indicated by the abbreviation “seq.”