Plasma ACE activity of the members of the affected family.

<p>ACE activity in heparinized plasma (1/5 dilution in PBS) of members of the affected family, the father (F), mother (M), and brother (B) as well as in 5 healthy individuals (marked by initials) that served as controls was determined by fluorimetric assay of 40 µl diluted plasma with 200 µl each of substrates Hip-His-Leu (HHL) and Z-Phe-His-Leu(ZPHL) during 1 hour of incubation. <b>A.</b> ACE activity with HHL: mean (± SD) of three independent determinations. <b>B.</b> Ratio of hydrolysis of the two substrates (<b>ZPHL/HHL ratio</b>) in the tested samples, which is used to characterize for each catalytic domain of ACE in somatic two-domain ACE <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010438#pone.0010438-Danilov3" target="_blank">[28]</a>. ND-not determined. * p<0.05 vs. control samples.