Enhancement of B19V transcription, replication and protein expression by CQ in UT7/Epo cells.

<p>Panel A shows the quantification of viral RNA synthesis 24 h after infection with B19V in the presence of increasing CQ concentrations. The results are the average of three independent experiments. SD bars are shown. Panel B shows the quantification of viral DNA replication at increasing post-infection times in untreated and CQ-treated (25 µM) cells. Values shown represent the average of two independent experiments. SD bars are shown. Panel C shows the kinetics of B19V structural protein expression in untreated and CQ-treated (25 µM) cells. Panel D shows the detection of viral protein expression by immunofluorescence in untreated and CQ-treated (25 µM) cells.