Gp96 deficiency induces an impaired phosphorylation of ERK and p38.

<p>A and B) Peritoneal macrophages were isolated from both WT and conditional KO mice and treated with lipopolysaccharides (LPS) (100 ng/ml) for 30, 60 and 120 minutes. C) The surface expression of CSF1R was analysed by flow cytometry in CD64+, F4/80+ peritoneal macrophages from both WT and conditional gp96-KO mice. The histograms are representative for one out of three independent experiments. D and E) Peritoneal macrophages were isolated from both WT and conditional KO mice, and treated with 10 ng/ml Macrophage Colony-stimulating Factor (M-CSF) for 3 days and restimulated with 10 ng/ml M-CSF for 10 minutes. Total protein was then isolated and Western blots for p-ERK, ERK, p-p38 and p38 were performed. “Vh” stands for macrophage treated with vehicle. Graphs show densitometric quantification of the blots (n = 5). In all cases data are expressed as mean±SEM. Statistical analysis was performed by One-way ANOVA followed by a Newman-Keuls test. ** = <i>P</i><0.01, *** = <i>P</i><0.001 vs respective non-treated macrophages and # = <i>P</i><0.05, ## = <i>P</i><0.01 vs M-CSF treated macrophages from WT mice.