miRNA mode of action is developmentally and temperature-dependent.

<p><i>(A) LUC mRNA expression levels assayed by qRT-PCR normalized to LUC mRNA in rLUC control plants (red dotted line)</i>. <i>Lines</i>, <i>(blue = 16</i>°<i>C</i>, <i>green = 23</i>°<i>C) represent the average between two biological replicates</i>. <i>(B) LUC protein levels</i>. <i>Black dots represent one biological replicate each calculated from two technical replicates</i>. <i>Lines</i>, <i>(blue = 16</i>°<i>C</i>, <i>green = 23</i>°<i>C) represent the average between two biological replicates normalized to LUC protein levels in rLUC control plants (red dotted line)</i>. <i>(C) Coexistence index is the ratio of average protein levels by average mRNA levels from each sample and condition</i>. <i>(D) AGO1 expression levels assayed by qRT-PCR</i>. <i>Black dots represent one biological replicate each calculated from two technical replicates</i>. <i>Lines</i>, <i>(blue = 16</i>°<i>C</i>, <i>green = 23</i>°<i>C) represent the average between two biological replicates</i>. <i>(E) AGO10 expression levels assayed by qRT-PCR</i>. <i>Black dots represent one biological replicate each calculated from two technical replicates</i>. <i>Lines</i>, <i>(blue = 16</i>°<i>C</i>, <i>green = 23</i>°<i>C) represent the average between two biological replicates</i>. <i>(A-E) “Inflores” stands for inflorescences</i>. * shows tissues in which temperature has a significant effect in a pairwise comparison (p<0.05). Letters and lines show significant differences between tissues in ANOVA-test after Tukey correction (adjusted p<0.05).