Additional file 3: Figure S1. of Down-regulation of ghrelin receptors on dopaminergic neurons in the substantia nigra contributes to Parkinson’s disease-like motor dysfunction

Detection of a selection marker cassette knock-in by PCR. Genotyping by PCR was performed by using the primers listed in Table S3. For the detection of alleles of wildtype or indels, 5’PARK2-PCR-Fw and 3’PARK2-PCR-Rv, PARK2-Exon2-PCR-Fw and PARK2-Exon2-PCR-Rv were used. A large 5082-bp and 3464-bp fragment derived from knock-in allele is also detectable in this primer set. For the detection of alleles of knock-in, 5’PARK2-PCR-Fw and PGKp-Rv for detection of the 5′ knock-in fragment, and PuroR-Fw and 3’PARK2-PCR-Rv for detection of the 3’knock-in fragment were used. (JPEG 429 kb