EBNA-LP mutants are defective at transforming B cells from cord blood.

<p><b>A.</b> Cell cycle profiles of CD19+sorted adult and cord B cells infected with WT^w or LPKO^w viruses. Plots show the DNA quantity per cell (from DAPI staining). Quantities associated with different cell cycle phases are indicated. <b>B.</b> Transformation efficiencies for each infection were calculated from two-fold dilutions of infected cells (see <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1006890#sec014" target="_blank">Methods</a>). These efficiencies were averaged for each virus group in each cell type across 3 (maternal–orange bar) or 5 (cord–blue bar) infections per virus. Black dotted lines indicate the sensitivity of the analysis for each virus–ie the calculated efficiency if only one well across all of the infections was positive. <b>C.</b> Outgrowth of LCLs from cord blood measured by counting the number of activated (ie large) cells. Cell count data are in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1006890#ppat.1006890.s019" target="_blank">S2 Data</a>.